Fig. 6.

Effects of marimastat and varespladib on Russell's viper venom-induced clotting in ROTEM. 10 μM marimastat ( A ) or varespladib ( B ) was mixed with 50 μg/mL Russell's viper venom in citrated whole human blood before the addition of Extem reagents and monitoring the level of clot formation over 60 minutes in ROTEM. The traces shown are representative of four separate experiments performed using blood obtained from four donors. The cumulative data are shown for specific parameters such as clot formation time, maximum clot firmness, and area under the curve (AUC) for full clot formed. Data represent mean ± SD ( n  = 4). The p -values (* p  < 0.05, ** p  < 0.01 and *** p  < 0.0001) shown were calculated by one-way ANOVA using GraphPad Prism. C—vehicle control; I—inhibitor alone; V—venom alone; V + I—venom + inhibitor. ^* Significance when venom-treated samples compared with the controls. ^{^} Significance when venom and inhibitor-treated samples compared with the venom-alone controls.