Materials

Name	Company	Catalog Number	Comments
0.5% Triton X-100	J.T.Baker	X198–07	Store at room temperature (RT)
1% DEAE-Dextran	MP Biomedicals	195133
10% Formalin solution, neutral buffered	Sigma-Aldrich	HT501128
Agar	Oxoid	LP0028
24-well Cell Culture Plate	Greiner Bio-one	662160
5% Sodium bicarbonate	Sigma Aldrich	S-5761
6-well Cell Culture Plate	Greiner Bio-one	657160
96-well Cell Culture Plate	Greiner Bio-one	655–180
African green monkey kidney epithelial cells (Vero E6)	ATCC	CRL-1586
Ami HT	Spectral Instruments Imaging
Aura Imaging Software 3.2.0	Spectral Instruments Imaging		Image analysis software
Bovine Serum Albumin (BSA), 35%	Sigma-Aldrich	A9647	Store at 4 °C
Cell culture grade water	Corning	25–055-CV
Dulbecco’s modified Eagle’s medium (DMEM)	Corning Cellgro	15–013-CV	Store at 4 °C
Anesthesia gas machine	Veterinary Anesthesia Systems, Inc.	VAS 2001 R
Fetal Bovine Serum (FBS)	Seradigm	1500–050	Store at −20 °C
Four- to six-week-old female K18-hACE2 transgenic mice	The Jackson Laboratory	34860
Graphpad Prism Version 9.1.0	GraphPad
Isoflurane	Baxter	1001936040	Store at RT
MARS Data Analysis Software	BMG LABTECH
MB10 tablets	QUIP Laboratories	MBTAB1.5	Store at RT
Nano-Glo Luciferase Assay Reagent	Promega	N1110	This reagent is used to measure Nluc activity. Store at −20 °C
Nunc MicroWell 96-Well Microplates	ThermoFisher Scientific	269620
Nunc MicroWell 96-Well Microplates	ThermoFisher Scientific	269620
Penicillin/Streptomycin/L-Glutamine (PSG) 100x	Corning	30–009-CI	Store at −20 °C
PHERAstar FSX	BMG LABTECH	PHERAstar FSX
Precelleys Evolution homogenizer	Bertin Instruments	P000062-PEV00-A
Soft tissue homogenizing CK14 – 7 mL	Bertin Instruments	P000940-LYSK0-A
T75 EasYFlask	ThermoFisher Scientific	156499
VECTASTAIN ABC-HRP Kit, Peroxidase	Vector Laboratories	PK-4002	ABC kit and DAB Peroxidase Substrate kit