Figure 3 – Administration of NRP1–4 blocks VEGF-A mediated increase in sodium currents.

(A) Schematic diagram showing the recording configuration for whole-cell patch clamp measurements of isolated DRG neurons. (B) Representative current traces obtained from DRG neurons following treatment with the indicated compound or protein. (C) Current-density voltage plot for each of the above conditions demonstrating that VEGF-A enhances sodium currents, and that this enhancement can be blocked by co-application of NRP1–4. Current density was obtained by normalizing the inward current measured at each voltage step to cellular capacitance. (D) Peak sodium current densities for the indicated treatment conditions indicating that treatment with NRP1–4 blocks the VEGF-A induced increase in current density. (E) Voltage dependent activation curves for the above treatment conditions showing that there is no significant change in this measure due to treatment with VEGF-A or NRP1–4. Vehicle was 0.1% DMSO dissolved in the culture media and external recording solution. VEGF-A applied at 1nM and NRP1–4 at 12.5 μM. Data points are mean ± SEM; N as indicated in panel A; statistical differences determined using one-way analysis of variance, which was performed because all data passed the D’Agostino-Pearson test for normality. Biophysical properties reported in Table S2.