Table 3.
Delivery of antagomirs in preclinical models of CVDs.
| Cardiovascular disease (species) | Targeting approach and delivery system (route) | Target miRNA | Findings | Ref. |
|---|---|---|---|---|
| Atherosclerosis (mouse) | Anti-αvβ3 integrin polymer NPs (IV) | miR-33a | Reduced lesion size and increased M2 macrophages | 116 |
| Atherosclerosis (mouse) | Lentivirus (RO) | miR-33a | Increased hepatic ABCA1 protein and increased plasma HDL | 110 |
| Atherosclerosis (human and bovine cells) | Ad (in vitro study) | miR-33a | Increased ABCA1 protein and ApoA1-mediated cholesterol efflux | 108 |
| Atherosclerosis (mouse) | Anti-VCAM1 liposomes (IV) | miR-712 | Reduced lesion size and Timp3 expression | 118 |
| MI (rat) | Anti-cardiac troponin I liposomes (IV) | miR-1 | Repressed arrhythmias | 120 |
| MI (mouse) | Anti-AT1R dendrimers (IV) | miR-1 | Reduced apoptotic cells, fibrosis, and infarct size | 100 |
| MI (pig) | Polymer NP (IC) | miR-92a | Enhanced angiogenesis | 123 |
| MI (rat) | Gelatin hydrogel microsphere sheet (local device) | miR-92a | Enhanced angiogenesis and cardiomyogenesis, reduced infarct size, and improved left ventricle function | 104 |
| MI (mouse) | EVs (IM) | miR-21 | Reduced fibrosis and improved cardiac function | 105 |
| MI (mouse) | AAV9 (IM) | let-7i-5p | Reduced infarct size and enhanced cardiomyocyte proliferation | 73 |
| Hypertension (rat) | LV (IV) | miR-20b | Reduced blood pressure, artery wall thickness, and vascular fibrosis | 127 |
| Cardiac hypertrophy (mouse) | AAV9 (IV) | miR-20b | Decreased left ventricle thickness | 72 |
| Cardiac hypertrophy (mouse) | Liposome-like NP (IV) | miR-23a | Reduced left ventricle thickness and improved cardiac function | 129 |
| Cardiac hypertrophy (mouse) | Cholesterol-coated polymer NP (IV) | miR-182 | Repressed cardiomyocyte growth and improved cardiac function | 132 |
AT1R: angiotensin II type 1 receptor; AAV: adeno-associated virus; Ad: adenovirus; CVD: cardiovascular disease; NP: nanoparticle; VCAM1: vascular cell adhesion molecule 1; IC: intracoronary; IV: intravenous; RO: retro-orbital; IM: intramyocardial; EVs: extracellular vesicles; MI: myocardial infarction.