Table 5.
An example 24-well plate setup for evaluating the ability of 12 candidate antidote oligonucleotides 1–12 (A1-A12) to reverse the binding of the E07 EGFR-targeting aptamer to EGFR on cells
1 | 2 | 3 | 4 | 5 | 6 | |
---|---|---|---|---|---|---|
A | Cells alone | Cells + C36 nontargeting control | Cells + E07 | Cells + A1 → E07 | Cells + A2 → E07 | Cells + A3 → E07 |
B | Cells + A4 → E07 | Cells + A5 → E07 | Cells + A6 → E07 | Cells + A7 → E07 | Cells + A8 → E07 | Cells + A9 → E07 |
C | Cells + A10 → E07 | Cells + A11 → E07 | Cells + A12 → E07 | |||
D |