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. 2023 Jun 13;4(3):102357. doi: 10.1016/j.xpro.2023.102357
Reagent Amount
Beads containing cDNA in Buffer EB (from step 44) 16 μL
NEBNext End Repair Buffer, 10× 2 μL
NEBNext End Repair Enzyme Mix 1 μL
E.coli ligase 1 μL
Total 20 μL