Figure 2.

Ripasudil blocked the Kupffer cell activation after hepatocyte transplantation

(A–F) F4/80 immunostaining of liver sections was used to reveal Kupffer cells at 3, 6, and 12 h after transplantation of mTmG hepatocytes. The percentage of hepatocytes colocalized with Kupffer cells was quantified at 3, 6, and 12 h after transplantation of mTmG hepatocytes. The inset in (C) and (E) is a higher-magnification view of the area in the red box of each subpanel. (G) The number of Kupffer cells was quantified and normalized to the hepatocyte number in liver sections. The scale bars represent 100 μM in (A)–(C). Data are expressed as the means ± SEMs of three independent experiments. n.s, no significance. ∗p < 0.05. ∗∗p < 0.01.