Figure 7.

Knockdown of CD59a abolished ripasudil-enhanced hepatocyte liver engraftment

(A) Protein bands of western blot analysis of CD59a expression by hepatocytes after transduction with AAV expressing CD59a shRNA. (B) Gray value analysis of (A). (C) Real-time PCR analysis of CD59a expression by hepatocytes after transduction with AAV expressing CD59a shRNA. (D) Costaining of C5b-9 and CD59a was performed on hepatocytes incubated with fresh serum after knock down of CD59a. (E) Quantitative analysis of C5b-9 positive hepatocytes in (D). (F) The liver engraftment of hepatocytes expressing CD59a shRNA2 was analyzed by bioluminescence imaging 3 days after cell transplantation. (G) Quantitative analysis of (F). The scale bars represent 100 μM in (D). Data are shown as the means ± SEMs of three independent experiments. ∗p < 0.05, ∗∗p < 0.01.