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Separation of H3N2a and H3N2b. (a) Chromatograms of 2-AA derivatized H3N2a and H3N2b in type II GM1 gangliosidosis human urine, plasma, CSF, and cat brain stem samples. H3N2a is not found in the cat with brain stem as an example. (b) Enhanced product ion spectra of [M+H]+ ions of 2-AA derivatized H3N2a, H3N2b, and 2 interfering peaks (peaks 1 and 3) show that they are isomeric pentasaccharides.
