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Synthesis of H3N2b and its internal standard and confirmation of H3N2b structure. (a) Synthesis of H3N2b and d6-H3N2b. (b) Chromatogram (detected by product ion scan) and product ion spectra of [M+H]+ ion of 2-AA derivatized synthetic H3N2b after purification with HPLC. (c) Chromatogram (detected by product ion scan) and product ion spectra of [M+H]+ ion of 2-AA derivatized H3N2b in GM1 gangliosidosis urine.
