Fig. 3.

Filtering of subcutaneous adipose non-alcoholic fatty liver disease (NAFLD) differentially expressed (DE) genes to select serum biomarker candidates (SBCs). To identify SBCs among the list of 953 adipose NAFLD DE genes, we selected the genes that were DE for NAFLD in adipose tissue but not in the liver, coded for secreted proteins, had moderate to high expression in adipose tissue, and had >10x higher expression in the subcutaneous adipose tissue than in the liver. These filters reduced the list of 953 total adipose DE genes across steatosis, fibrosis, and non-alcoholic steatohepatitis (NASH) to 10 SBCs. Blue genes are upregulated in steatosis, fibrosis, and/or NASH in adipose tissue, while red genes are downregulated.