FIG. 5.

Micrographs of an infected sand fly at 6 days postfeeding. (A) UV fluorescence micrograph showing absence of apparent infection in head, thorax, and thoracic midgut (100 × magnification; scale bar = 3000 μm). (B) Anterior midgut showing rusty-brown bloodmeal by phase-contrast microscopy. The SV at the anterior is arrowed. (C) Corresponding fluorescence micrograph showing heavy colonization by GFP⁺ B. ancashensis below the SV and outside the PM but still within the confines of the anterior midgut (400 × magnification; scale bars = 100 μm in B, C). UV, ultraviolet.