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. 2023 Jun 5;18(6):1305–1314. doi: 10.1021/acschembio.3c00028

Figure 1.

Figure 1

Incorporation of ACK and AC2K into proteins in mammalian cells. (A) Chemical structures of HCK, HC2K, ACK, and AC2K. The photolabile group/fluorophore is colored blue. (B) Confirmation of ACK and AC2K incorporation into a reporter construct through fluorescence imaging in HEK293T cells (10× magnification) and western blot. (C) Expression of NLS-EGFP-AC2K in live NIH 3T3 cells (20× magnification). (D) Fixed HeLa cells expressing NLS-EGFP-ACK (top row, 40×) and NLS-EGFP-AC2K (bottom row, 63×). Cells are counterstained with rhodamine–phalloidin.