KEY RESOURCES TABLE.
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
|
| ||
| Antibodies | ||
|
| ||
| Guinea pig polyclonal anti-PLIN5 | Progen | GP44 |
| Rabbit polyclonal anti-PLIN2 | (Atshaves BP et al., 1999)72 | N/A |
| Mouse polyclonal anti-OXPHOS cocktail | Abcam | ab110413 |
| Rabbit polyclonal anti-Acadvl | Abcam | ab188872 |
| Rabbit monoclonal anti-Acadm | Abcam | ab92461 |
| Rabbit polyclonal anti-Acaa2 | Abcam | Ab128911 |
| Mouse monoclonal anti-Cs | Cell Signaling | 14309 |
| Rabbit monoclonal anti-Aco1 | Abcam | Ab183721 |
| Mouse Monoclonal anti-Idh3 | Abcam | ab154886 |
| Mouse Monoclonal anti-Suclg1 | Abcam | ab97867 |
| Mouse Monoclonal anti-Fh | Abcam | Ab233394 |
| Mouse Monoclonal anti-Mdh2 | Abcam | Ab181873 |
| Rabbit Polyclonal anti-Catalase | Cell Signaling | 14097S |
| Rabbit Polyclonal anti-COXIV | Cell Signaling | 4850S |
| Rabbit Polyclonal anti-Calreticulin | Cell Signaling | 12238S |
| Mouse Polyclonal anti-VAPB | ThermoFisher | MA5–24318 |
| Rabbit Polyclonal anti-Cpt1-a | Protein Tech | 15184–1-AP |
| Rabbit Polyclonal anti-BiP | Cell Signaling | C50B12 |
| Rabbit Polyclonal anti-MIGA2 | ThermoFisher | PA-5–71096 |
| Rabbit Polyclonal anti-SLC25a1 | Thermo | 15235–1-AP |
| Rabbit Polyclonal anti-FAS | Cell Signaling | 3180S |
| Rabbit Polyclonal anti-ACSL1 | Abcam | Ab189939 |
| Rabbit Polyclonal anti-ACSL5 | Santa Cruz | SC-47999 |
| Rabbit Polyclonal anti-FADS6 | Novus | NBP2–83031 |
| Rabbit Polyclonal anti-AGPAT2 | Cell Signaling | D8W9B |
| Donkey anti-Guinea pig IRDye 800CW | LI-COR | Cat No. 926–32411 |
| Donkey anti-Guinea pig IRDye 680RD | LI-COR | Cat No. 926–68030 |
| Donkey anti-Rabbit pig IRDye 800CW | LI-COR | Cat No. 926–32213 |
| Donkey anti-Rabbit pig IRDye 680RD | LI-COR | Cat No. 926–68022 |
| Donkey anti-Mouse pig IRDye 800CW | LI-COR | Cat No. 925–32212 |
| Donkey anti-Mouse pig IRDye 680RD | LI-COR | Cat No. 926–68023 |
|
| ||
| Bacterial and virus strains | ||
|
| ||
| DH5a | Invitrogen | 18258–012 |
| XL1-Blue | Agilent | Cat No. 200150 |
| Stable | NEB | C3040H |
| pLV-Bsd-TRE-SplitGFP (SPLICSs- MT-ER)-CMV-rtTA | This paper | N/A |
|
| ||
| Chemicals | ||
|
| ||
| Trypsin Gold, Mass Spec Grade | Promega | V5280 |
| MitoTracker Deep Red | Invitrogen | M22426 |
| [14C]oleate | Perkin Elmer | NEC317250UC |
| FCCP | Abcam | Ab120081 |
| TMRE | Abcam | Ab113852 |
| Etomoxir | Abcam | Ab254445 |
| [13C]palmitate | Sigma-Aldrich | 687871–3.80G |
| Ponceau S | Fisher BioReagents | BP103–10 |
| AutoDOT-Neutral lipid Stain | Abcepta | SM1000a |
| BODIPY-C12FL | Invitrogen | D3821 |
| Halotag reagent JF646 | Promega | GA1120 |
| [14C]acetate | American Radiolabeled Chemicals, Inc. | ARC0173 |
| Analtech Preadsorbent Silica gel HL Plates | Miles Scientific | P43911 |
| Percoll | Sigma | P4937–100ML |
| 2-APB | Tocris | 1224 |
| Clotrimazole | Tocris | 4096 |
| ADP | Sigma-Aldrich | A5285 |
| Oligomycin A | Sigma-Aldrich | 75351 |
| Rotanone | Sigma-Aldrich | R8875 |
| Antimycin A | Sigma-Aldrich | A8674 |
| Malonate | Sigma-Aldrich | 63409 |
| Duroquinol | Sigma-Aldrich | D223204 |
| TMPD | Sigma-Aldrich | T7394 |
| Ascorbate | Sigma-Aldrich | A4034 |
| Sodium Azide | Sigma-Aldrich | S8032 |
|
| ||
| Critical commercial assays | ||
|
| ||
| TMT-10plex | ThermoFisher | 90110 |
| Seahorse kit | Agilent | 103015–100 |
| Effectene | QIAGEN | 301425 |
|
| ||
| Deposited data | ||
|
| ||
| CM-PDM Control-PLIN5 knockdown proteomics | MassIVE:https://massive.ucsd.edu/ProteoSAFe/static/massive.jsp | ftp://massive.ucsd.edu/MSV000091575/ |
| CM-MAM-PDM-MAM Control-PLIN5 knockdown proteomics | MassIVE:https://massive.ucsd.edu/ProteoSAFe/static/massive.jsp | ftp://massive.ucsd.edu/MSV000091576/ |
|
| ||
| Experimental models: Cell lines | ||
|
| ||
| AML12 | ATCC | CRL-2254 |
|
| ||
| Experimental models: Organisms/strains | ||
|
| ||
| Male C57BL6/J mice | Envigo | N/A |
|
| ||
| Oligonucleotides | ||
|
| ||
| Control Anti-Sense Oligonucleotide | Ionis Pharmaceuticals; Mark Graham | N/A |
| Perilipin5 Anti-Sense Oligonucleotide | Ionis Pharmaceuticals; Mark Graham | N/A |
|
| ||
| Recombinant DNA | ||
|
| ||
| pSEMS-TOM20-Hal7Tag | Addgene | 111135 |
| pLV-Bsd-TRE-SplitGFP(SPLICSs- MT-ER)-CMV-rtTA |
This paper | N/A |
| pEZ-M29-mCherry-mPLIN5 | GeneCopoeia | EX-mm27089-m29 |
| p-SPLICSs-(Split-GFP ER-Mito Sensor) | CieriDetal. (2017)41 | N/A |
| pLV-Bsd-TRE-CMV-rtTA | Addgene | 128061 |
|
| ||
| Software and algorithms | ||
|
| ||
| SigmaPlot 11 | Systat Software, Inc | N/A |
| Prism8 | GraphPad | N/A |
| Canvas 11 | Canvas | N/A |
| Avizo 3D Software | Thermo Fisher Scientific | N/A |
| Image Studio v5 | LI-COR Biosciences | N/A |
| NIS-Elements 4 | Nikon | N/A |
| Microsoft Photos Application | Microsoft | N/A |
| HAL4000 | Zhuang lab | Github: https://github.com/ZhuangLab/storm-control |
| Insight3 | Xiaowei Zhuang lab | Github: https://github.com/BoHuanglab |
| Cross-correlation-filtering | Puchner Lab | Github: https://github.com/PuchnerLab |
| MATLAB | MathWorks | MATLAB |
| Diana-FIJI | Gilles JF et al. (2017)43 | N/A |
| Scaffold 5 | Proteome Software | Scaffold 5 |