Extended Data Figure 2. BCKDK muscle knockout is specific and does not affect body weight or muscle physiology on chow diet.

a-d, Gene expression data via qPCR of Bckdk, Bckdk exon 6 & 7 (deleted region), Bckdha, and Bckdhb, respectively; n=3 Bckdk^l/l (WT) and n=5 Bckdk^l/l-HSA-CreER (Cre). e, Body weights; n=8 WT and n=8 Cre. f-j, Western quantification of BCKDK in fasted tissues normalized to loading control; n=3 WT and n=3 Cre. k-o, Western quantification of the ratio pBCKDH/BCKDH in fasted tissues; n=3 WT and n=3 Cre. p, Western blotting for p-ACLY and ACLY in fasted livers; n=6 WT and n=6 Cre. 14-3-3 is the loading control. q, Western quantification of the ratio p-ACLY/ACLY from the blot in p. r, H&E staining of tibialis anterior (TA) muscle. Representative images from n=4 WT and n=3 Cre mice. s, Ratio of 3-HIB/valine, and t, Concentration of 3-HIB in the plasma from mice fasted for 5-hr (n=10 WT and n=8 Cre) or re-fed for 2-hr after an overnight fast (n=10 WT and n=8 Cre). Mice used for these experiments were male, aged 8–10 weeks and fed chow diet. Comparisons of two groups use two-tailed Student’s t-test with significance defined as: *p<0.05, **p<0.01, ***p<0.001.