Extended Data Figure 8. DBT liver knockout does not affect body weight or glucose tolerance on multiple diets or after a BCAA challenge.

a-c, Western quantification of DBT in tissues normalized to loading control; n=3 Dbt^l/l-AAV8:GFP (GFP) and n=3 Dbt^l/l-AAV8:Cre (Cre). d, Body weights of n=10 GFP and n=10 Cre mice on chow and HFD. e, Plasma labeling of BCAAs after U¹³C-BCAA gavage (fraction); n=4 GFP and n=5 Cre. Labeling fraction of TCA cycle intermediates 10 min post U¹³C-BCAA gavage in f, quad g, iBAT, & h, gWAT in 5-hr fasted mice; n=4 GFP and n=5 Cre. i, BCKDH complex activity measured in fasted livers from n=4 GFP and n=4 Cre mice. j, 1.5g/kg glucose IP-GTT in 5-hr fasted mice fed HFD for 4 weeks after a BCAA gavage given at t=0; n=8 GFP and n=9 Cre. k, Area-under-the-curve (AUC) for the GTT in j. l, Insulin concentration at t=0 and t=20 min (n=8 GFP and n=9 Cre) during GTT from j. m, Plasma BCAA concentration in 5-hr fasted mice before (t=0) and 20 min after (t=20) a gavage of BCAAs from GTT + BCAA gavage in j; n=8 GFP and n=9 Cre. Mice used in these experiments were male, aged 16–24 weeks and fed a chow diet for 4–5 weeks, followed by a HFD for 4–6 weeks. Comparisons of two groups use two-tailed Student’s t-test with significance defined as: *p<0.05. Experiments with multiple comparisons at different time-points use two-way ANOVA with repeated measures.