Fig. 2 |. Increased SM BCAA oxidation does not affect insulin sensitivity in mice fed normal chow.

a, Western blotting of tissues collected from n = 3 fasted Bckdk^loxP/loxP and n = 3 fasted Bckdk^loxP/loxP-HSA-CreER mice (14-3-3 is the loading control). b, H&E staining of quadricep muscle. Representative images from n = 4 Bckdk^loxP/loxP and n = 3 Bckdk^loxP/loxP-HSA-CreER mice. c, Plasma BCAA concentrations in mice fasted for 5 h (n = 9 Bckdk^loxP/loxP and n = 8 Bckdk^loxP/loxP-HSA-CreER) or refed for 2 h after overnight fasting (n = 10 Bckdk^loxP/loxP and n = 8 Bckdk^loxP/loxP-HSA-CreER). d, Plasma BCKA levels in fasted or refed mice (n = 7 Bckdk^loxP/loxP and n = 7 Bckdk^loxP/loxP-HSA-CreER). e, IP-GTT (2 g kg⁻¹) using n = 8 Bckdk^loxP/loxP and n = 8 Bckdk^loxP/loxP-HSA-CreER chow-fed mice fasted for 5 h. f, AUC for the GTT in e. g, Insulin concentration at t = 0 and t = 20 min (n = 8 Bckdk^loxP/loxP and n = 8 Bckdk^loxP/loxP-HSA-CreER mice) during the GTT from d. The mice used for these experiments were male, 8–10-weeks old and fed normal chow. Data are presented as the mean ± s.e.m. When two groups were compared, a two-tailed Student’s t-test was used, with significance defined as *P < 0.05 and **P < 0.01. In the experiments with multiple comparisons at different time points, a repeated measures, two-way ANOVA was used. c, Valine fasted P = 0.006624; leucine fasted P = 0.012123; isoleucine fasted P = 0.006569. d, KIC/KMV fasted P = 0.000837; KIV fasted P = 0.000859.