Fig. 6 |. Increased liver BCAA oxidation does not affect insulin sensitivity in mice fed HFD.
a, Western blotting of tissues from n = 3 BckdkloxP/loxP-AAV8-GFP and n = 3 BckdkloxP/loxP-AAV8-Cre mice. Liver BCKDK is the bottom band, as shown by the black arrow. The top band is non-specific (14-3-3 is the loading control). b, Plasma BCAA concentration in mice fasted for 5 h or refed for 2 h after overnight fasting; n = 8 BckdkloxP/loxP-AAV8-GFP and n = 10 BckdkloxP/loxP-AAV8-Cre. c, Plasma BCKAs in mice fasted for 5 h; n = 4 BckdkloxP/loxP-AAV8-GFP and n = 5 BckdkloxP/loxP-AAV8-Cre. d, Liver pool size of BCAAs. e, Liver pool size of BCKAs. f, Labelling fraction of TCA cycle intermediates 10 min after gavage of [U-13C]-labelled BCAAs in mice fasted for 5 h; n = 4 BckdkloxP/loxP-AAV8-GFP and n = 5 BckdkloxP/loxP-AAV8-Cre. g, IP-GTT (1.5 g kg−1) in mice fasted for 5 h and fed HFD for 4 weeks; n = 8 BckdkloxP/loxP-AAV8-GFP and n = 10 BckdkloxP/loxP-AAV8-Cre. h, AUC of the GTT in g. i, Insulin concentration at t = 0 (n = 8 BckdkloxP/loxP-AAV8-GFP and n = 10 BckdkloxP/loxP-AAV8-Cre) and t = 20 min (n = 8 BckdkloxP/loxP-AAV8-GFP and n = 9 BckdkloxP/loxP-AAV8-Cre) during the GTT in g. j, IP-GTT (1.5 g kg−1) in mice fasted for 5 h and fed HFD for 2 weeks; n = BckdkloxP/loxP-AAV8-GFP and n = 10 BckdkloxP/loxP-AAV8-Cre. k, AUC of the GTT in j. l, IP-GTT (1.5 g kg−1) in mice fasted for 5 h and fed HFD for 2 months; n = 9 BckdkloxP/loxP-AAV8-GFP and n = 10 BckdkloxP/loxP-AAV8-Cre. m, AUC of the GTT in l. Mice used in these experiments were male, 12–21-weeks old and were fed HFD for 4–12 weeks. Data are presented as the mean ± s.e.m. When two groups were compared, a two-tailed Student’s t-test was used, with significance defined as *P < 0.05. In the experiments with multiple comparisons at different time points, a repeated measures, two-way ANOVA was used with significance defined as *P < 0.05. d, Valine P = 0.039715; leucine P = 0.024237; isoleucine P = 0.030860. g, 20 min P = 0.0348.