Extended Data Figure 1. BT2 treatment, acutely or chronically, does not affect body weight.

a-c, Western quantification of the ratio p-BCKDH/BCKDH in fasted tissues; n=6 vehicle- and n=6 BT2-treated. d, Plasma BCAA concentration in HFD-fed mice fasted for 5-hr or re-fed for 2-hr after an overnight fast; n=10 vehicle- and n=10 BT2-treated. Mice were gavaged with 50mg/kg/day of either vehicle or BT2 for 4 weeks prior to plasma collection. e, Body weights of mice used for acute BT2 experiment; n=10 vehicle- and n=10 BT2-treated. f, Body weights of mice used for chronic BT2 experiment; n=10 vehicle- and n=10 BT2-treated. g, Body weights of mice used for chronic BT2 GTT (at treatment day 28); n=10 vehicle- and n=10 BT2-treated. Mice used for these experiments were male C57BL/6J diet-induced obesity (DIO) mice ordered from the Jackson Laboratory. They were age 10–12 weeks and on a HFD for 4–6 weeks. Comparisons of two groups use two-tailed Student’s t-test with significance defined as: ***p<0.001. Experiments with multiple comparisons at different time-points use two-way ANOVA with repeated measures.