Table 1. Different preparations of drug free NLC and SEPI-loaded NLC .

Formulation	Lipid (%w/v)				Surfactant (%w/v)				Particle size (nm)	PDI	Z-potential (mv)
	GPS		GMS	BW	CP	POL188	SP80	Glucire
Non SEPI	1	3.5	-	-	-	2.5	-	-	138.5 ± 2.34	0.166 ± 0.030	-26.8 ± 4.35
	2	3.5	-	-	-	5	-	-	111.8 ± 3.21	0.227 ± 0.08	-27.8 ± 3.21
	3	-	1.75	1.75	-	-	0.75	1.75	140.0 ± 2.98	0.179 ± 0.05	-21.1 ± 4.67
	4	-	1.75	1.75	-	-	1.5	3.5	85.09 ± 3.31	0.262 ± 0.07	-20.7 ± 5.31
	5	-	-	-	3.5	-	0.75	1.75	186.4 ± 4.04	0.170 ± 0.06	-20.9 ± 3.56
0.4 % w/v SEPI	6	3.5	-	-	-	2.5	-	-	172.0 ± 4.43	0.235 ± 0.01	-19 ± 1.5
	7	3.5	-	-	-	5	-	-	180.1 ± 5.01	0.232 ± 0.03	-20.6 ± 2.86
	8	-	1.75	1.75	-	-	0.75	1.75	128.2 ± 3.99	0.189 ± 0.02	-22.2 ± 1.6
	9	-	1.75	1.75	-	-	1.5	3.5	68.41 ± 3.34	0.287 ± 0.02	-22.1 ± 2.1
	10	-	-	-	3.5	-	0.75	1.75	214.1 ± 2.45	0.363 ± 0.03	-39.3 ± 1.7

* In all formulation, MCT oil 1.5% was used as liquid lipid and the volume of water was 10 mL.