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. 2023 Jun 18;21:396. doi: 10.1186/s12967-023-04217-0

Fig. 1.

Fig. 1

The binding affinity and specificity of aptamer to CD40. A The predicted secondary structure of CD40 aptamer (CD40Apt) using mfold program. B Equilibrium dissociation constants (Kd) of CD40Apt to TAO mouse orbital fibroblasts, 293 T and HUVEC were detected by Flow cytometry. C Kd in CD40 knocked-down TAO mouse orbital fibroblasts and CD40 overexpressed 293T were detected by Flow cytometry. D Aptamer pull-down assay was applied to detect the binding between CD40Apt and CD40 in mouse orbital fibroblasts