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. 2023 Jun 19;20:126. doi: 10.1186/s12985-023-02085-0

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — MVA-induced neutralizing antibodies against MVA or MPXV. BALB/c mice (n = 5) were immunized twice with different MVA concentrations. (A) On day 42 postimmunization, the neutralizing antibody titers against MVA virus induced by MVA were determined by PRNT with 100 TCID₅₀ MVA virus on BHK-21 cells. (B) On day 42 postimmunization, the neutralizing antibody titers against MPXV induced by MVA concentration of 10⁷ TCID₅₀/mL were determined by PRNT with 600 pfu MPXV on Vero cells. Comparing the vaccination effect among MVA and PBS groups, the asterisk * indicates a significant difference (P < 0.05) detected by one-way ANOVA or T-test analysis.