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. 2023 Jun 19;21:147. doi: 10.1186/s12964-023-01156-7

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© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Effect of HQ in combination with 5-FU on CRC cell proliferation. H630R1 (A), RKOR10 (B) and MC38 (C) cells were treated with various concentrations of HQ and 5-FU for 72 h. Cell viability was measured by WST-1 assay (left panel). The Combination-Index (CI) was calculated with CI < 1 indicating synergism (right panel) from at least 3 individual experiments performed in duplicate