Figure 2.

Liver sections stained for CCasp3, stripped, and stained with H&E from a mouse treated with 5 mg/kg HFPO-DA. (A) Two possibly necrotic hepatocytes based on features in Figure 1A (H&E stain). (B) Same section stained for CCasp3. (C) A putative necrotic hepatocyte surrounded by inflammatory cells based on examples in Figure 1B-C (H&E stain). (D) Same section stripped and stained for CCasp3. Source: Mouse 5017 from DuPont OECD 421. ⁸ CCasp3, cleaved caspase-3; H&E, hematoxylin and eosin; HFPO-DA, ammonium 2,3,3,3-tetrafluoro-2-(heptafluoropropoxy)-propanoate.