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. 2023 Apr 9;12(11):12569–12580. doi: 10.1002/cam4.5907

FIGURE 1.

FIGURE 1

Generation and characterization of anti‐GM2 CAR‐T cells expressing IL‐7, CCL19, and herpes simplex virus‐thymidine kinase. (A) Schematic representation of Conv. CAR and 7 × 19 CAR against GM2. LS; leader sequence, TM; transmembrane region. (B) Human PBMC transduced with Conv. CAR or 7 × 19 CAR were stained with anti‐idiotype Ab against anti‐GM2 scFv to detect CAR expression, along with anti‐CD8 Ab. UTD T cells were examined as a negative control. The percentage of cells in each quadrant is indicated. (C) The culture supernatants from Conv. CAR‐T and 7 × 19 CAR‐T cells were harvested 4 days after gene transduction, and the concentrations of IL‐7 and CCL19 were measured by ELISA. As a control, the culture supernatant obtained from UTD T cells at the same time point was examined. Data are shown as mean ± standard error of triplicate samples. N.D., not detected.