FIGURE 5.

Generation of target‐specific long‐term memory responses by the treatment with 7 × 19 CAR‐T cells. NOG‐ΔMHC mice were inoculated subcutaneously (s.c.) with 1 × 10⁷ Lu‐135 cells on day 0, followed by treatment with intravenously injection of 1 × 10⁷ 7 × 19 CAR‐T cells on day 3. (A) The mice with complete tumor regression were rechallenged s.c. with 1 × 10⁷ Lu‐135 tumor cells at the right flank and 5 × 10⁶ SW480 tumor cells at the left flank on day 28. As a control, naïve NOG‐ΔMHC mice were inoculated s.c. with Lu‐135 and SW480 tumor cells in the same fashion (n = 6 in the tumor‐rejected mice group, n = 4 in the naïve NOG‐ΔMHC mice group). (B) The mice with a complete tumor regression were rechallenged s.c. with 3 × 10⁶ KMS11 tumor cells at the right flank and 5 × 10⁶ SW480 tumor cells at the left flank on day 35. As a control, naïve NOG‐ΔMHC mice were also inoculated s.c. with KMS11 and SW480 tumor cells in the same fashion (n = 4 in the tumor‐rejected mice group, n = 5 in the naïve NOG‐ΔMHC mice group). Tumor size was measured twice a week by a digital caliper. Tumor volumes are shown as mean ± standard error. ***p < 0.001, n.s., not significant.