Figure 1.

EMSA with a radiolabelled telomeric C-rich strand (27h) and HeLa nuclear extracts. The probe (1 µM 27h) was incubated in 50 mM HEPES buffer pH 7.2, containing 0.1 M KCl, 1 mM MgCl₂ and 5% sucrose (15 min on ice) and run at 4°C on an 8% polyacrylamide gel (see Materials and Methods). Lane 1, 27h probe alone; lanes 2–5, HNE 0.9 µg/µl. The competitors dT26 (lanes 3 and 5) and ds26 (lanes 4 and 5) were added at 0.5 µg/µl each. A, B, C, K and D refer to the names of the bands used in the text.