Figure 2.

(Upper) EMSA with the 27h probe and increasing concentrations of HeLa nuclear extracts. The radiolabelled probe (10 nM) was incubated in 50 mM HEPES buffer pH 7.2, containing 0.1 M KCl, 1 mM MgCl₂ and 5% sucrose, in the presence of ds26 and dT26 (0.5 µg/µl each) and various concentrations of HNE for 15 min on ice and run at 4°C on an 8% polyacrylamide gel (see Materials and Methods). Lane 1, no protein; lanes 2–10, increasing concentrations of HNE (1.7, 4.25, 8.5, 17, 42.5, 85, 170, 425 and 850 ng/µl). (Lower) Quantification of the radioactivity in the different bands divided by the total radioactivity in the lane. Closed circle, free probe; closed diamond, band D; open circle, band K; open diamond, band C.