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. 2000 Apr 1;28(7):1564–1575. doi: 10.1093/nar/28.7.1564

Figure 5.

Figure 5

Partial characterisation of the C-rich strand-binding proteins. (A) EMSA with a fluorescein labelled C-rich strand telomeric oligonucleotide (F26) in a 2% vertical agarose gel. Incubation in MES buffer and migration were performed at 4°C (see Materials and Methods). Fluorescence was revealed with a UV table (312 nm). Lane 1, F26 alone; lane 2, F26 + HNE; lane 3, HNE. (B) Agarose slices cut from the previous EMSA corresponding to the retarded band and to the control lane were loaded on an 8–16% SDS–PAGE gel. The gel was then silver stained. Lane 1, molecular weight marker; lane 2, F26 + HNE from the EMSA; lane 3, HNE from the EMSA. (C) Southwestern analysis on a duplicate of the gel presented in (B). Hybridisation overnight with labelled 27h in 50 mM MES pH 6.0, containing dT26 (10 µg/ml) at 4°C. Lane 1, crude HNE; lane 2, F26 + HNE from the EMSA; lane 3, HNE from the EMSA.