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. 2000 Apr 1;28(7):1647–1655. doi: 10.1093/nar/28.7.1647

Figure 4.

Figure 4

Expression of RecQ5 isoforms in 293EBNA cells. (a) Schematic representations of three recombinant RecQ5 helicase isomers, RecQ5α, RecQ5β and RecQ5γ, all N-terminally tagged with influenza virus 6xHA. RecQ5βc contains an N-terminal 6xHA tag and the C-terminal region of RecQ5β (amino acid residues 746–991). Numbers indicate amino acid residues. (b) Western blot analysis of three RecQ5 helicase isomers and RecQ5βc expressed in 293EBNA cells. Cell lysates prepared from cells transiently transfected with each construct were size fractionated by SDS–PAGE and analyzed by western blot analysis using anti-HA antibody.