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. 2000 Apr 1;28(7):1647–1655. doi: 10.1093/nar/28.7.1647

Figure 6.

Figure 6

Co-immunoprecipitation of RecQ5β and human topoisomerases 3α and 3β. (a) Expression of 6xHA-tagged RecQ5β protein in 293EBNA cells. 293EBNA cells were transfected with 6xHA–RecQ5β, Flag–Top3α and Flag–Top3β as indicated. Cell lysates were prepared from the cells, size fractionated by SDS–PAGE and analyzed by western blot analysis using anti-HA antibodies. (b) Expression of Flag-tagged type I topoisomerase proteins in 293EBNA cells. Anti-Flag antibodies were used in a western blot analysis. (c) RecQ5β protein co-immunoprecipitated with topoisomerases 3α and 3β. Type I topoisomerases and their binding proteins were precipitated with anti-Flag IgG-conjugated agarose beads and were eluted with Flag peptide. The eluted proteins were size fractionated by SDS–PAGE and analyzed by western blot analysis using anti-HA antibodies.