| No. of cycles | Step | Temperature | Time |
|---|---|---|---|
| 1 | Initial denaturation | 95°C | 2 min |
| 45 | Denaturation | 95°C | 30 s |
| Annealing | 54°C | 40 s | |
| Extension | 68°C | 40 s | |
| 1 | Final extension | 68°C | 10 min |
| 1 | Hold | 4°C | ∞ |
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