Table 1.
Taxon name, voucher number, and DNA concentration for the 13 samples collected and preserved in 96% ethanol. The DNA concentrations correspond to the DNA extracts prepared from each sample after three weeks, six weeks, and four years. Misplaced values are absent for the first two samples at the three‐week time point.
| Sample taxon | Voucher | DNA concentration after 3 wk (ng/μL) | DNA concentration after 6 wk (ng/μL) | DNA concentration after 4 ya (ng/μL) |
|---|---|---|---|---|
| Causonis japonica (Thunb.) Raf. | IB 2010‐082 | NA | 4.7 | <0.01 |
| Tetrastigma lawsonii Herb. Kew | Wen 11680 | NA | 5.0 | <0.01 |
| Pterisanthes eriopoda (Miq.) Planch. | Wen 11831 | 4.2 | 2.7 | 42.3 |
| Tetrastigma rafflesiae Planch. | Wen 11824 | 37.2 | 28.3 | 710.0 |
| Ampelocissus ascendiflora Latiff | Wen 11822 | 4.4 | 1.3 | 550.0 |
| Cissus discolor Blume | IB 2012‐011 | 4.9 | 0.9 | 301.0 |
| Cyphostemma juttae (Dinter & Gilg) Desc. | IB 2010‐093 | 4.8 | 2.8 | 407.0 |
| Cyphostemma sandersonii (Harv.) Desc. | IB 2010‐094 | 6.7 | 2.8 | 183.0 |
| Cissus tuberosa Moc. & Sessé ex DC. | IB 2010‐091 | 13.4 | 10.2 | 214.0 |
| Vitis monticola Buckley | Wen 12662 | 2.1 | 3.4 | 137.0 |
| Vitis munsoniana J. H. Simpson ex Planch. | Wen s.n. | 4.1 | 11.0 | 174.0 |
| Parthenocissus heptaphylla (Planch.) Britton | Wen 12657 | 4.2 | 7.7 | 71.8 |
| Nekemias arborea (L.) J. Wen & Boggan | Wen 12694 | 9.0 | 16.0 | 291.0 |
a
After four years of storage, the DNA was extracted using proteinase K in the lysis buffer and a doubled amount of input tissue was processed.