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. 2023 Apr 29;4(4):zqad020. doi: 10.1093/function/zqad020

Figure 1.

Figure 1.

Muscle structure is altered in Pcyt2+/- mice. (A) Images and weights of quadricep muscle of Pcyt2+/- relative to Pcyt2+/+ showing enlarged quadricep muscle. (B) Hematoxylin and eosin staining of the quadriceps show intramuscular lipid accumulation and degenerating myofibers. Black asterisks show lipid accumulation, blue arrows show smaller, hypereosinophilic fibers with several cells exhibiting the internal migration of nuclei, black arrow shows a swollen hypereosinophilic cell. (C) Transmission electron microscopy of the quadriceps. Orange asterisks show disordered sarcomere structure with loss of proper striation, black arrows show swollen and irregular mitochondria, and red arrows show increased presence of vacuoles. (D) Images of hindlimb clasping in Pcyt2+/- and (E) quantification clasping using a scoring system based on the degree and length of time the limbs are retracted toward the abdomen. Pcyt2+/- mice show increased hindlimb clasping, which is indicative of muscle weakness or neuromuscular disorders. Data are derived from both male and female mice and presented as mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.