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. 2023 Apr 29;4(4):zqad020. doi: 10.1093/function/zqad020

Figure 2.

Figure 2.

Increased macrophage infiltration and fibrosis. Images of IHC staining of the quadricep using (A) Anti-CD3 antibody to determine T-cell infiltration and (B) Anti-F4/80 antibody to determine macrophage infiltration in Pcyt2+/−relative to Pcyt2+/+ quadricep muscle. Immunohistochemistry shows normal CD3 + cell content but an increased presence of F4/80 + macrophages in Pcyt2+/- indicating cellular injury and inflammation. (C) Western blot analysis in the quadricep shows elevated content of proinflammatory p-p38 Mapk normalized to p38 Mapk (p-p38 Mapk: p38 Mapk), and p65 NfκB and Stat3 normalized to Gapdh. (D) Staining using Masson’s trichrome for collagen deposition (blue) in the quadricep showing increased fibrosis in Pcyt2+/−. Data are derived from both male and female mice and are presented as mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.