Figure 2.

Increased macrophage infiltration and fibrosis. Images of IHC staining of the quadricep using (A) Anti-CD3 antibody to determine T-cell infiltration and (B) Anti-F4/80 antibody to determine macrophage infiltration in Pcyt2^+/−relative to Pcyt2^+/+ quadricep muscle. Immunohistochemistry shows normal CD3 + cell content but an increased presence of F4/80 + macrophages in Pcyt2^+/- indicating cellular injury and inflammation. (C) Western blot analysis in the quadricep shows elevated content of proinflammatory p-p38 Mapk normalized to p38 Mapk (p-p38 Mapk: p38 Mapk), and p65 NfκB and Stat3 normalized to Gapdh. (D) Staining using Masson’s trichrome for collagen deposition (blue) in the quadricep showing increased fibrosis in Pcyt2^+/−. Data are derived from both male and female mice and are presented as mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.