Figure 2.

NU9056 inhibits survival and proliferation in human ATC cells. Endogenous KAT5 protein (a) and mRNA (B) expressions were detected by Western blot or qRT-PCR in human ATC (8505C and CAL-62) and normal thyroid (Nthy-ori 3–1) cells. Then, CAL-62 cells were left untreated or treated with NU9056 (2.5–40uM) for 48h. Then, the expression levels of acetyl-histone H2A, total histone H2A, acetyl-histone H4, and total histone H4 were detected by Western blot (C). Next, 8505C (D, E), CAL-62(F, G), and Nthy-ori 3–1(H, I) cells were left untreated or treated with NU9056 (2.5–40uM), cells were further cultured in indicated time periods, and then, cell viability (D) F, (H) and proliferation (E) G, (I) were tested by the appropriate assays. Cal-62 cells transiently transfected with either control or KAT5 shRNA. The knockdown effect was detected by RT-PCR (J) or Western blot analysis (K), cells were left untreated or treated with NU9056 (2.5–40uM), cells were further cultured in indicated time periods, and then, cell viability (L) was tested by the appropriate assay. ^∗p < 0.05 vs. “Ctrl” group, ^∗∗∗p < 0.01 vs. “Ctrl” group.