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. 2023 May 25;30(6):740–752. doi: 10.1038/s41594-023-00999-5

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 1 — a, Pat1 activity tethered to 3′ UTR of both fluorescent reporters with PP7 is reproducible between replicates and fluorophores. b, Schematic representation of Sgn1 recruiting Pab1 and eIF4G in the tethering assay. c, Comparison of RFP and YFP fluorescence with Sgn1 or a non-regulator control tethered to YFP (n = 3, one representative replicate depicted). d, YFP fluorescence with Sgn1 and the non-regulator Halo protein tethered to the 3′ UTR. e, RFP fluorescence with Sgn1 and the non-regulator Halo protein tethered to the 3′ UTR of YFP. f, Forward scatter fluorescence of Sgn1 and control protein expressing cells indicates larger cell size in the Sgn1 samples. g, RT-qPCR analysis of YFP mRNA levels with Sgn1 or the control tethered to the 3′ UTR.