Fig. 2.
Limit of detection for correct allele calling of 42 Deeplex Myc-Lep markers. The LOD was evaluated with DNA extracts from three M. leprae strains and an 85-15% mixture of two strains. (Top) Read depth at core and non-core markers versus the number of RLEP copies. Median (line) and mean (grey dot) values as well as 25–75% quartiles are shown. (Bottom) For each serial dilution with 3.103, 3.104, 3.105 and 3.106 RLEP copies (80-80,000 M. leprae genomes), the fraction of correctly (green) and incorrectly detected or not sequenced (grey) alleles was determined for 144 core and 24 non-core alleles (36 core and six non-core markers times four DNA extracts, respectively).