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Limit of detection in terms of the minimum proportion of detectable minority variants, using M. leprae reference strains (VNTR markers not considered). Mixes of the susceptible strain NHDP63 with 1, 5, 10, 20, 50 and 80% of the resistant strain Br14-3 were used to estimate the lowest fraction of detectable variant allele. The resistant strain was also analysed alone, as a control (100%, bottom). The resistant allele is depicted in red while the susceptible allele is in grey.
