Fig. 4.

Limit of detection, in terms of RLEP qPCR Cq value, for the sequencing of 36 Deeplex Myc-Lep core markers (including the species identification target), determined by using 213 biopsies from patients affected by leprosy. (Top) Read depth at Deeplex Myc-Lep core markers versus RLEP PCR Cq values 13–30. (Bottom) Proportion of successfully sequenced Deeplex Myc-Lep core markers versus RLEP PCR Cq values 13–30 (Top & Bottom) Median (line) and mean (grey dot) values as well as 25–75% quartiles are shown.