Table 3.
Comparison of Deeplex Myc-Lep and VNTR-FLA core and non-core VNTR analysis results from 89 M. leprae clinical samples.
| VNTR | Total Tested | Match | Partial Match | ND | Mismatch | %Match |
|---|---|---|---|---|---|---|
| 6-3a | 89 | 86 | 0 | 3 | 0 | 100 |
| AC8a | 89 | 75 | 0 | 14 | 0 | 100 |
| AC8b | 89 | 86 | 0 | 3 | 0 | 100 |
| AC9 | 89 | 82 | 0 | 6 | 1 | 98.8 |
| GTA9 | 89 | 79 | 1 | 9 | 0 | 100 |
| GAA21 | 89 | 82 | 1 | 4 | 2 | 97.6 |
| GGT5 | 89 | 86 | 0 | 3 | 0 | 100 |
| 12–5 | 89 | 84 | 0 | 5 | 0 | 100 |
| 21–3 | 89 | 76 | 1 | 12 | 0 | 100 |
| 23–3 | 89 | 70 | 0 | 19 | 0 | 100 |
| 6–7 | 89 | 65 | 0 | 24 | 0 | 100 |
| 18–8 | 31 | 26 | 0 | 5 | 0 | 100 |
| 27–5 | 31 | 29 | 0 | 2 | 0 | 100 |
| Total | 1041 | 926 | 3 | 109 | 3 | 99.5 |
Non-core VNTR markers 18-8 and 27-5 were sequenced using 250bp paired-reads on a subset of 31 samples. Match, same VNTR marker allele detected by Deeplex Myc-Lep and VNTR-FLA; partial match, two alleles detected by one of the methods, including one matching with the other method; ND, not detected by Deeplex Myc-Lep and/or VNTR-FLA; mismatch, Deeplex Myc-Lep and VNTR-FLA detected different VNTR marker alleles.