Table 3.

BTK and PLCG2 nonhotspot mutations in relapsed and responsive cohorts, as assessed by targeted NGS analysis

Patient ID	Cohort	Time point	Gene	coding DNA description	protein description	protein domain	NGS VAF (%)	References	Other BTK hotspot mutations	Other PLCG2 hotspot mutations
18	Relapsed	relapse	BTK	c.136C>T	p.R46C	PH	2.6	none	yes	no
26	Relapsed	relapse	BTK	c.946A>G	p.T316A	SH2	3.0	Sharma et al 2016; Kadri et al 2017; Jones et al 2017	no	yes
				c.1003G>C	p.V335L	SH2	3.6	none
34	Responsive	≥1 y	BTK	c.1283C>A	p.A428D	TK	6.0	Wang et al 2022	yes	yes
7-PBMC	Relapsed	relapse	PLCG2	c.601C>G	p.Q201E	EF	2.7	none	yes	no
1	Relapsed	relapse	PLCG2	c.683T>A	p.F228Y	EF	1.7	none	yes	yes
6	Relapsed	relapse	PLCG2	c.2543T>G	p.L848R	PH	9.3	Landau et al 2017	yes	yes
60	Responsive	≥1 y	PLCG2	c.846G>C	p.E282D	EF	3.8	none	no	no
83	Responsive	≥1 y	PLCG2	c.3076A>T	p.A1026T	Y	3.2	none	no	no