Table 3.

Binding parameters of the interaction of FV mutants and immobilized EGR-APC or PS in an SPR-based assay (BIAcore)

	EGR-APC immobilized				PS immobilized
FV mutant	kass	kdiss	Kd	Chi2	kass	kdiss	Kd	χ2
	×105 M−1s−1	×10−3 s−1	nM		×105 M−1s−1	×10−3 s−1	nM
WT	3.37	3.59	10.7	1.29	5.20	3.93	7.56	1.76
A2086D	1.32	2.68	20.3	0.824	9.84	1.93	19.6	0.919
W1920R	3.38	4.33	12.8	0.832	1.45	3.82	26.2	0.708
R506Q	2.39	4.57	19.1	0.106	7.11	3.74	5.26	0.960

Reactions were performed are described in “Materials and methods.” Parameter values were calculated by nonlinear regression analysis from data in Figure 4A-B using the evaluation software provided by Biacore AB.

EGR-APC, EGR active site-blocked APC.