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. 2023 Jun 19;10:29. doi: 10.1186/s40580-023-00377-3

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 12 — Label-free characterization of intrahepatic fat accumulation in liver steatosis, using near-infrared MSOT. a Clinical MSOT images with ultrasound-aided ROI delineation of subcutaneous fat (SAT) and the liver. b Preclinical MSOT imaging with cryoimaging-aided ROI selection of SAT and the liver. Comparative normalized ratio spectra, and normalized liver ROI/background mean pixel intensity ratio at 930 nm for c for the whole participant cohort (steatotic, n = 5; healthy, n = 5), and d for the whole animal model cohort (high fat diet, n = 5; regular chow diet, n = 5). The images are reproduced with permission from [173]