Table 2.

Summary of ITC sequential binding studies data

PEAK3 peptide, or peptide complex (peptide sequence)	ITC expt. type	CrkIINSH3				14-3-3γ
		SPR KD (µM)	N	ITC KD (µM)	N	SPR KD (µM)	N	ITC KD (µM)	N
PEAK354–66 peptide alone (PLPPPLPKKILTR)	Binary	1.7 ± 0.4	3	ND	–	No binding	3	ND	–
PEAK3tandem, 54–74 peptide alone (PLPPPLPKKILTRTQSLPTRR)	Binary	0.60 ± 0.02	2	0.60	1	No binding		No binding
PEAK3tandem, 54–74:14-3-3γ complex (PLPPPLPKKILTRTQSLPTRR)	Ternary	ND		0.70	1	NA		NA
PEAK3tandem, 54–74pS69 peptide alone (PLPPPLPKKILTRTQpSLPTRR)	Binary	2.2 ± 0.7	2	1.1	1	0.19 ± 0.04	4	0.130 ± 0.7	4
PEAK3tandem, 54–74pS69:14-3-3γ complex (PLPPPLPKKILTRTQpSLPTRR)	Ternary	ND		3.6 ± 0.6	4	NA		NA

Refer to Supplementary Data 3–5 (SPR, ITC data). Values are mean, uncertainties are SD (N = 2) or S.E.M (N ≥ 3), from the listed number of independent experiments (N). Peptide residues from relevant consensus sequence motifs are bolded.