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(a) 13C/13C 2D projection of a 1H-detected (H)CCH 3D spectrum of 13C- and 15N-labeled Aβ42 fibril. (b) Enlarged view of spectral region extracted from (a), showing the signals corresponding to Cβ-Cα-Hβ correlations, for comparison with the Cβ-Cα assignments of Aβ42 fibrils with known S-shaped structure. (c) A simulated spectrum from the 13Cβ-13Cα assignments of the Aβ42 fibril with S-shaped structure.27
