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. 2023 May 24;20:100683. doi: 10.1016/j.mtbio.2023.100683

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© 2023 The Authors. Published by Elsevier Ltd.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 3 — (A) Double staining of cytoskeleton and nucleus of mBMSC after co-cultivation with TS and TS@PAP implants for 48 h. Confocal microscopy images for TS at 10x (a, scale bar: 50 μm) and 40x (c, scale bar: 20 μm) magnification and for TS@PAP at 10x (b, scale bar: 50 μm) and 40x (d, scale bar: 20 μm) magnification. (B) In vitro short-term PTX release in solutions with pH values of 7.4 and 6.5. (C) In vitro long-term release of free PTX from TS@PAP implants after incubation at a pH of 6.5. (D) Cell activity of co-cultured mBMSC with TS and TS@PAP implants (n = 5, ∗P < 0.05). (E) Confocal microscopy images of live-dead staining of mBMSC after co-cultivation with TS (a, scale bar: 500 μm) and TS@PAP (b, scale bar: 500 μm) implants for 48 h.