Skip to main content
. 2023 Jun 19;13(6):e1300. doi: 10.1002/ctm2.1300

FIGURE 6.

FIGURE 6

LncRNA DUXAP8 directly binds to SLC7A11 and promotes lysosomal sorting. (A) Binding of SLC7A11 and DUXAP8 detected by RIP assay. (B) SLC7A11 was abundantly enriched in biotinylated DUXAP8 precipitates as detected by silver staining and WB assay. (C) Half‐life of SLC7A11 protein in LM3 cells with and without DUXAP8 knockdown. Cycloheximide (CHX), 100 mg/mL. (D) Effects of MG‐132 (20 μM) and bafilomycin A1 (Baf A1, 10 μM) treatment for 6 h on SLC7A11 expression in LM3 cells with and without DUXAP8 knockdown. (E) DUXAP8 knockdown reduced the distribution of SLC7A11 (green) in lysosomes (marked by LAMP2, magenta). The scatter diagram was conducted by using the Image Pro Plus software. * p < 0.05, **p < 0.01, ***p < 0.001. NS, not significant. Results represent three independent experiments. sh‐DUX, sh‐DUXAP8; EV, Empty vector; OE, pcDNA DUXAP8.