Table 2.
Soluble amino acid contents in dry seeds of the EV, four seed-specific lines expressing the RNAi::AtMGL (A); wild-type (WT) and the mgl mutant (B). wild-type (WT) and the mgl mutant.
| (A) | (B) | |||||||
|---|---|---|---|---|---|---|---|---|
| AAs | EV | Si2 | Si3 | Si6 | Si8 | WT | mgl | |
| Alanine | 94.5 ± 8 | 83.9 ± 4.1 | 71.7 ± 7.4 * | 67.3 ± 2.9 * | 65.1 ± 0.01 * | 94.2 ± 23.8 | 71.9 ± 10.1 | |
| Valine | 58.7 ± 7.9 | 45.3 ± 9.8 | 21.9 ± 9.8 * | 24.9 ± 7.9 * | 22.2 ± 0.01 * | 62.3 ± 41.4 | 55.6 ± 32.3 | |
| Serine | 93.4 ± 6.2 | 71.5 ± 6 * | 64.3 ± 11.7 * | 66 ± 4.7 * | 66.1 ± 0.01 * | 99 ± 65 | 111 ± 114.8 | |
| Leucine | 22.6 ± 4.4 | 13.7 ± 2.2 * | 9.2 ± 0.8 * | 7.4 ± 0.4 * | 8.6 ± 0.003 * | 30.9 ± 15.6 | 23.1 ± 15.4 | |
| Threonine | 55.4 ± 11.9 | 53.9 ± 6.2 | 53.9 ± 6.2 | 44.7 ± 7.1 | 32.2 ± 0.02 * | 84.2 ± 25.4 | 109.5 ± 40.5 | |
| Isoleucine | 24.5 ± 4.5 | 10.6 ± 2.8 * | 7.3 ± 1.4 * | 7.9 ± 0.9 * | 7 ± 0.01 * | 37.6 ± 6.8 | 6.9 ± 2.1 | |
| Glycine | 67.8 ± 3.9 | 45.6 ± 8.5 * | 45 ± 9.6* | 40.5 ± 5.7 * | 54.9 ± 6.4* | 63.2 ± 30.2 | 70.5 ± 47.9 | |
| Homoserine | 9 ± 3.2 | 9.2 ± 0.9 | 7.8 ± 1 | 6.9 ± 1.3 | 6.3 ± 0.02 | 9.2 ± 6.3 | 14.1 ± 4.1 | |
| Methionine | 11.7 ± 2.5 | 30.7 ± 5.3 * | 24.2 ± 5.2 * | 29.7 ± 2.1 * | 25.9 ± 0.001 * | 13.5 ± 3.9 | 22.3 ± 0.3 * | |
| Aspartate | 153.3 ± 35.2 | 79.1 ± 13.8 * | 67.4 ± 11 * | 65.3 ± 2.6 * | 63.1 ± 0.002 * | 153.8 ± 121.8 | 73.1 ± 67.3 | |
| Phenylalanine | 59.2 ± 8 | 68.8 ± 4.3 | 65.3 ± 4.9 | 86.3 ± 4.2 * | 67.3 ± 0.4 | 59.3 ± 13.3 | 60.5 ± 18.7 | |
| Glutamate | 404.1 ± 59.5 | 345.1 ± 87.8 | 212.4 ± 33.2 * | 260.2 ± 47.4 * | 263.4 ± 11.5 * | 405 ± 101.6 | 348.3 ± 77.4 | |
| Homocysteine | 13.2 ± 1.1 | 12.8 ± 1.1 | 12.5 ± 1.1 | 13.6 ± 2.6 | 13.3 ± 2.1 | 13.1 ± 2.7 | 11.9 ± 1.1 | |
| Asparagine | 161.5 ± 19.4 | 151.8 ± 28 | 118.7 ± 23.4 | 176 ± 22.8 | 139.5 ± 72.9 | 72 ± 4.3 | 62.7 ± 4.5 * | |
| Tyrosine | 58.1 ± 6.3 | 83.6 ± 14.8 * | 83.1 ± 8.7 * | 100.8 ± 5.3 * | 102 ± 14 * | 709 ± 32.6 | 731.9 ± 62 | |
The seeds were developed on plants undergoing slight heat stress of 27°C. The content was examined in dry seeds using GC-MS.*
*The amounts of amino acids were calculated based on norleucine that was used as an internal standard. The results are mean ± SD of five replicates. Student’s T-test was used to determine statistically significant differences (p<0.05) between the EV and each of the RNAi lines, as identified by an asterisk. The seeds of WT and mgl were examined at a different time from the other seeds of the RNAi lines/EV.