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. 2023 May 8;32:794–806. doi: 10.1016/j.omtn.2023.05.004

Figure 1.

Figure 1

Kinetics of Cy5-Fluc mRNA fluorescence and expression after i.m. delivery via different LNPs

(A) Representative IVIS images of cyanine-5 fluorescence signal in mice injected with 5 μg of naked cyanine-5-labeled, 5- methoxyuridine-modified CleanCap FLuc mRNA or the same mRNA encapsulated within MC3, KC2, or L319 LNPs. (B) Quantification of the fluorescence signal by total radiance efficiency (AU = (ph/s)/(μW/cm2)) in the injected site at 0, 2, 4, 6, 24, 48 and 72 h post injection. (n = 4 to 5 mice per group). Data are presented as mean values +/− SEM. (C) Representative bioluminescence images of luciferase expression in the Cy5-Fluc mRNA-injected mice. (D) Quantification of the bioluminescence signal (AU = photons/s) in the injected site at 6, 24, 48 and 72 h post injection. (n = 4 to 5 mice per group). Data are presented as mean values +/− SEM.